FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22375544
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG137600.057.45203894
CAAGTCG100050.029.03148594
TAAGTCG37700.027.9257394
GAAGTCG145850.023.68529394
GCGAAAT28300.019.0989381
AAAGTCG54500.018.11009294
TTTTTTC364800.015.7182022
GTTTTTT271200.015.6320071
TTTTTTG389850.014.5273832
CGAAATA22050.014.4943312
GAAAAAT199800.014.161161
GCCGAAT61500.013.83252051
AAAAATC190800.013.0555552
GCGGAAT46300.012.4859611
AAATCCA184550.012.4280684
GGAAAAT153050.012.2835681
AATCCAC108900.012.127645
CGCGAAC15950.012.0815051
GTCGGTT45700.012.0328241
GCCGATT25900.011.9768341
Produced by FastQC (version 0.11.5)