FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22375544
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG174850.045.10494694
CAAGTCG125000.029.177694
TAAGTCG37950.025.14097494
GAAGTCG146250.019.44273694
TATACGC10350.018.1642517
TACGCTA10900.016.385329
AAAGTCG70750.014.01696194
GCGAAAT28650.013.7801051
GTTTTTT263400.013.7574021
CTTTAAC70050.013.0835111
GCCGAAT57900.012.9067351
CGCAAAC46650.012.6945341
AAATCCA183300.012.5641024
ATATACG14600.012.5547956
GTTTTCT186650.012.4896871
GTTTAAT81700.012.4834771
CGAAAAC42300.012.4444451
AAATCGA31900.012.3761764
CGCAAAT33950.012.3210611
CTCGATT44800.011.9598221
Produced by FastQC (version 0.11.5)