FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22389193
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114450.054.3302794
CAAGTCG87400.029.5228894
TAAGTCG33900.026.61946794
GAAGTCG127350.021.95916694
GTTTTTT301850.015.6952131
CGAAAAT35550.015.6005611
AAAGTCG46200.015.463201594
TTTTTTC372800.013.7167382
GTCGATT17850.013.1652661
TATACGC10150.012.9655177
GAAAAAT215600.012.8617811
TTTTTTG399600.012.7262262
GTTTAAT93350.012.536691
TACGCTA11050.012.3348419
AAAAATC206450.012.31629852
CGCAAAT32750.012.0549611
GTTTTCT218050.011.9628521
CGAAAAC36650.011.926331
AATCCAC108950.011.2592935
GCGAAAT27000.011.1407391
Produced by FastQC (version 0.11.5)