FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21872468
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127800.053.47261494
CAAGTCG99050.030.27360294
TAAGTCG34200.026.38596594
GAAGTCG135100.019.2731394
AAAGTCG48100.017.88149894
TACGCTA8600.016.9418629
TATACGC9150.014.8961757
ATATACG12700.013.6929146
CGCGAAT13650.013.08424951
TTAGCGA21350.012.768157
CGAAAAT31500.012.5333341
GTCGGTT48250.012.4683941
GTTTTTT267850.012.1075241
GTTTATT97900.011.8580191
CGCGGAT23850.011.82391
CGAAGAT83700.011.679811
CGGAAAT47800.011.4058591
CGCGTAT4559.505351E-611.36263751
GTTTTAC60100.011.0266231
GTCGGAT31800.010.7893091
Produced by FastQC (version 0.11.5)