FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22538169
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG141750.051.1280494
CAAGTCG119400.029.52261294
TAAGTCG34550.025.98263294
GAAGTCG132600.020.80618394
AAAGTCG55900.013.70482994
GTTCGAT30100.012.1794011
AATCGTA21650.012.1570445
GTCCGAT14650.010.9078491
TTAGCGA20150.010.7295278
GGGAAAT145600.010.5879121
CGCGTAA8803.1832315E-1010.1477271
TATACGG16250.010.12307634-35
TTCGATT33550.010.0864372
GTCGTAT9802.0008883E-1110.0714271
CGTATCG17850.010.0056023
GGCGAAT40600.09.8399011
GTCGTTT20700.09.7632851
GCGGTAT23250.09.7032251
TCGGATT26700.09.6816472
CGGGAAT54200.09.5387451
Produced by FastQC (version 0.11.5)