FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23804120
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG121350.055.54017394
CAAGTCG87050.027.37392294
TAAGTCG36250.024.50482794
GAAGTCG136000.020.11323594
GTTTTTT342450.015.2343411
AAAGTCG55350.014.94489694
CGAAAAT37400.014.3262021
TTTTTTC431550.013.8642112
CGAATCG18150.012.9476593
GCGAAAT34000.012.4411761
TTTTTTG472250.012.2812072
CGCAAAT30600.012.1339861
GTTTAAT110150.011.9900131
CGCGATT8250.011.9636361
GAAAAAT265650.011.8539431
AATCCAC132000.011.3583345
AAATCCA222200.011.3375344
GTTTTAT186950.011.1623431
CGGAAAT52750.011.1374411
CTCGATT44000.011.1090911
Produced by FastQC (version 0.11.5)