FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22378140
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG155100.046.1818294
CAAGTCG119500.027.4133994
TAAGTCG33900.026.48082594
GAAGTCG135900.020.8888994
AAAGTCG63200.015.914556594
GCGAAAT28550.015.1453591
CGCGTAT4001.469216E-714.0999991
TTAGCGA17850.013.9551817
CGCTAAT9150.013.8688521
CGGAAAT41850.013.7013151
CGAAAAT50300.012.9880711
GTTTTTT286350.012.8189281
GCCGAAT54000.012.7074071
TAGCGAG20300.012.5024628
CTCGATT36950.012.4654941
CGCAAAC47900.012.4613781
AATCGGG40700.011.8943495
CCGAATC67450.011.8458122
AATCCAA97200.011.7983545
AAATCCA185100.011.781744
Produced by FastQC (version 0.11.5)