FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22004923
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG70500.035.494
CAAGTCG58850.017.33050294
TAAGTCG20800.016.94711594
GTTTATT106000.016.272641
CGCAATT19050.014.8031491
CTTTATT174700.014.3663431
TAGGCTC104850.013.17882694
GTTTTAT158900.013.0440531
GTCGGTT53550.012.989731
CGAATCG12450.012.83534053
GTCATTT133650.012.8005981
GTTTTAC64550.012.5964371
GTTTAAT89300.012.4736841
GTCCGAT9850.012.4060921
CGGAAAT41100.012.3503661
CGAAAAT33600.012.3095241
GGAAAAT181300.012.23607351
AATCGGG47300.012.1226225
GTTTTTT292900.012.002731
GTTTTCT225250.011.8934521
Produced by FastQC (version 0.11.5)