FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22743709
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG110750.056.8243894
CAAGTCG88650.028.78849494
TAAGTCG37250.023.08993594
GAAGTCG120450.021.69530994
CGCGAAT15200.017.934211
GTTTTTT313150.016.3295541
AAAGTCG54700.016.3254194
GCGAAAT28950.015.7478411
GCGGAAT40400.015.3564371
TTTTTTC451100.014.9720692
CGCAAAT26100.013.86591
CGAAAAC37650.013.8565741
AAATCCA214300.013.2907144
TTTTTTG470400.012.8390732
GTTTAAT107900.012.8063021
AAATCGA32800.012.7530494
AAAAATC250350.012.6910312
GAAAAAT257950.012.5722051
AATCCAC122450.012.3209485
CGAAAAT38150.012.3197921
Produced by FastQC (version 0.11.5)