FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22743709
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG135650.046.22041794
CAAGTCG101500.026.90344894
TAAGTCG35800.022.05586494
GAAGTCG120000.019.66166794
GTTTTTT320500.014.89921951
CTCGATT38700.014.4521971
GCCGATT23000.014.0999991
CGCTAAT10250.013.7560981
GCCGAAT54700.013.7477151
AAAGTCG62150.013.53660594
CGGATAT24450.013.4560321
GCGAAAT26900.013.4535321
CGCGTTT14350.012.77351951
AAATCCA212900.012.4950694
CGCGAAT12300.012.2276421
CGAATCG15400.012.2077933
CGAAAAC40300.012.1290311
TATACGC8950.012.0782127
TACGCTA9400.012.09
AAAAATC248700.011.73582652
Produced by FastQC (version 0.11.5)