FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21982084
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG119650.048.63017394
CAAGTCG91200.027.88048694
TAAGTCG32450.023.6086394
GAAGTCG130050.019.98539294
CGAAAAT32650.015.6906591
AAAGTCG45550.013.92974894
GTCGGTT46000.013.4869581
TACGCTA8400.013.4285729
TATACGC7800.012.6538477
GTTTATT101150.012.1741
CGAATCG14300.011.8321693
CGAATCC40000.011.7500013
GCCGAAT45100.011.5676281
CGGAAAT47750.011.5162311
GGCGAAT54300.011.5119711
GGAAAAT175900.011.4360441
GCGGTAT23650.011.3276961
CGAAGAT81350.011.323911
TTTACGC41500.011.2120497
CTCGGAT65900.010.8406691
Produced by FastQC (version 0.11.5)