FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21982084
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG140750.037.60000294
CAAGTCG104700.025.04871294
TAAGTCG32900.023.57142894
GAAGTCG119450.017.70615494
GTCGGAT40600.014.12315494
AAAGTCG57350.012.78465794
CGAAGAT75150.012.4457771
GTAAAAC64250.012.1431911
GGCGAAT39100.011.6598461
TCGGATC42100.011.49881494
AATCGTA16000.011.456255
TATACGC7401.6916601E-1011.4324337
GTCGGTT42650.011.3505281
CGAATCG14500.011.3448293
GGAAAAT169500.011.2578181
TACGCTA7602.6921043E-1011.1315799
CGAAAAC34750.010.9553971
CGCAAAT31750.010.8062991
CGGAAAT43100.010.7958251
TAGCGAG23850.010.641519
Produced by FastQC (version 0.11.5)