FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23194839
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG85000.045.0647194
CAAGTCG67400.023.77893394
TAAGTCG24400.020.99590394
GTTCGAT28400.019.031691
TTCGATT34050.014.7694572
GAAGTCG113550.014.36283694
TATACGG19200.013.46354234-35
GTCGAAT13000.013.0153841
TCGGATT28500.012.5333332
TCGATTC39450.011.9138153
CGCGTAA5505.7084253E-711.1090911
GTATACG20150.010.96277834-35
CTCGGAT64800.010.5169751
GTCCGAT16400.010.3170741
CTCGGTT38250.010.1986921
TACCGTA22600.010.1902669
AATCGTA23100.010.1731615
GTCGGAA43000.010.1651161
GTCGTTT22500.010.0266661
CGCTTAA30050.010.00998344-45
Produced by FastQC (version 0.11.5)