FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23194839
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG105050.036.5083394
TAAGTCG27150.020.7734894
CAAGTCG81250.020.36184794
GAAGTCG109950.013.42246494
GTTCGAT28150.013.3570161
CGCGTAT5754.474714E-1013.0782611
CGCGTAA5705.487891E-912.3684221
GTCGTTT22600.012.2699111
TTCGATT32750.012.1984732
GTCCGAT15900.012.1194971
GTCGGTT50650.011.2280361
CGCGGAT22250.011.1955051
AAAGTCG48650.010.4337194
CTCGGTT39900.010.3659141
TATACGG17600.010.2812534-35
TTAGCGA22500.010.0266668
CGTATCG20000.09.873
GTTTATT118650.09.784241
CGATTGG18750.09.5253334
CGCGAAT10406.91216E-119.4903851
Produced by FastQC (version 0.11.5)