FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22182966
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG120450.056.85263494
CAAGTCG95450.030.2336394
TAAGTCG35800.026.2569894
GAAGTCG131650.022.52715594
AAAGTCG51600.018.6724894
TACGCTA8500.014.3764719
CGAATCG14850.012.976433
CGCGAAT7850.012.5732491
CGAAAAT31800.012.5628921
CGAAGAT86100.012.1730541
ATATACG10550.012.0284366
TATACGC7853.6379788E-1211.9745237
GTTTATT113250.011.6203081
AATCGTA17800.011.6179775
GGCGAAT50100.011.2574841
CAATCGG29750.011.2168054
GTTTTAT154500.011.1644011
AATCGGG40350.010.9491945
GTTTTTT298950.010.8951321
CGGAAAT44000.010.6818181
Produced by FastQC (version 0.11.5)