FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22182966
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG138550.045.49043394
CAAGTCG106050.026.72418494
TAAGTCG35400.025.75706194
GAAGTCG125000.019.777694
AAAGTCG59250.014.9130894
CGGAAAT42650.013.6647121
TACGCTA9200.013.2826089
CGCGAAT6903.6379788E-1212.9420281
TATACGC9150.012.841537
GTCGGTT36200.011.6850821
CGCAAAC42250.011.5692311
CGAAAAT41000.011.348781
GTTTATT113850.011.2700911
CGAAGAT77250.011.1948211
CGCGTAT5052.4742258E-611.1683161
GGAAAAT183300.010.794871
CTCGGAT61100.010.769231
CTTTAAT108750.010.7613791
CGCGTTT16600.010.7590351
ATATACG12850.010.6070026
Produced by FastQC (version 0.11.5)