FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22174383
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG129650.056.80601594
CAAGTCG102800.029.3064294
TAAGTCG35300.025.83002794
GAAGTCG137700.023.61946394
AAAGTCG51150.017.27468194
TTTTTTC375450.016.3739512
GTTTTTT293600.015.7200261
GCCGAAT53500.013.9682241
CTCGATT34400.013.5261631
GAAAAAT215850.013.5218891
AAATCCA198700.013.506294
GCGAAAT26150.013.300191
TTTTTTG411700.012.9686662
CGAAAAT33500.012.9074621
AAAAATC211350.012.8980352
GGAAAAT159900.012.5509691
GTCGATT20850.012.3980821
CGAAAAC32500.012.2923071
CGCAATT21550.012.2134571
GTTTAAT88550.012.1547151
Produced by FastQC (version 0.11.5)