FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22174383
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG153750.047.99349294
CAAGTCG120150.026.67831894
TAAGTCG35750.023.66433594
GAAGTCG134400.022.55580394
AAAGTCG60250.016.84979294
CGAAAAC32450.015.2080111
GTTTTTT290950.014.9262741
GCCGAAT47950.013.5265891
CGCGAAT7000.013.4285711
CTCGATT37500.013.1599991
CGAAAAT41850.012.2413381
CGCAATT20650.012.0629541
GGAAAAT160500.011.8890961
GTCGGAT38750.011.88645194
GTTTTTC159900.011.7573481
GTTTAAT87700.011.7366021
GAAAAAT207250.011.7244861
CTCAGAT163750.011.538321
CGCGAAC12700.011.472441
AAATCCA200050.011.4651344
Produced by FastQC (version 0.11.5)