FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21860992
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG95850.051.87897594
CAAGTCG75100.028.53794794
TAAGTCG30600.022.5784394
GAAGTCG116250.021.34709594
GTTTTTT283450.016.5648251
TTTTTTC358800.014.9331092
AAAGTCG45400.014.07929594
AAAAATC209600.013.7681292
AAATCCA201250.013.4986324
GAAAAAT215300.013.4254521
CGGATAT23500.013.1999991
CTCGATT34150.013.0746691
CGAAAAT36250.012.5765511
GTCGAAT13300.012.368421
AAAATCC182200.012.0724473
GTTTTAC68300.012.0424591
CGAATCG15700.011.9745233
CGAAAAC33050.011.8033281
AATCCAC107900.011.760895
GTTTTTC162100.011.742751
Produced by FastQC (version 0.11.5)