FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21860992
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124900.040.1136994
CAAGTCG92250.024.5571894
TAAGTCG34100.022.74193494
GAAGTCG117350.019.26459394
GCGAAAT25200.015.6666661
GTTTTTT272050.015.0130491
TATACGC8350.014.6347297
TACGCTA10000.014.0999989
CGCGAAT7850.013.77071
CGAATCG14650.013.4744023
CGCAAAC42550.013.3654521
AAATCGA32350.013.221024
CGCAAAT31300.012.9137381
CGAAAAT42400.012.7476421
CGGATAT24150.012.6501041
CGGAAAT44600.012.645741
AATCGTA21250.012.6070585
CTCGATT38800.012.5979381
AAAGTCG53450.012.22263794
CGAAAAC35300.012.1161471
Produced by FastQC (version 0.11.5)