FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22557871
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG154100.046.87800694
CAAGTCG110550.026.0615194
TAAGTCG44800.023.81473494
GAAGTCG135950.019.1180694
AAAGTCG63500.015.691339594
CGCGTAT5101.5454134E-812.9019611
CGAAGAT85750.012.6064141
GTTTTAT151650.011.8391051
CGCAAAC42700.011.7775181
GTCCGAT16150.011.6408671
CTCGTTT41150.011.4216291
GTTTTTT267200.011.2047171
CGGAAAT47150.011.164371
CGAATCG16850.011.157273
CGCGTTT13500.011.140741
CGCAAAT29800.010.882551
GGCGAAT36250.010.7613791
CGAAAAT43450.010.7088611
GGAAAAT192900.010.4525661
AATCGTA20400.010.3676475
Produced by FastQC (version 0.11.5)