FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22535342
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG129650.037.26648794
TAAGTCG29800.022.08053894
CAAGTCG95550.020.6593494
GAAGTCG123850.016.1283894
CGCGTAT4008.893039E-915.2750011
TACGCTA7900.014.2784819
CGGAAAT43300.013.351041
CGAAAAC33950.012.7363781
GTCGGTT46400.012.1551721
TATACGC7057.2759576E-1112.07
AAATCGA33850.011.8020684
AATCGGG40250.011.6770185
GGAAAAT169850.011.4559911
CGAAGAT86700.011.3840841
GTTTTTT263300.011.370681
CTTTAAT96500.011.2020721
GTTTAAT77100.011.0946831
AATCCAA99350.010.8807255
CGCGGTT47100.010.7770691
AATCGTA17450.010.7736395
Produced by FastQC (version 0.11.5)