FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21622067
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106150.053.6194194
CAAGTCG80550.027.42396494
TAAGTCG31200.025.15705394
GAAGTCG124750.020.9098294
TATACGC7750.016.9806467
GTTTTTT260350.016.6986751
TACGCTA7100.016.5492979
TTTTTTC321350.016.0298752
AAAGTCG44200.014.88687894
AAAAATC190700.013.8757222
GCGAAAT31550.013.7052311
AAATCCA186150.013.6594154
GAAAAAT193350.013.5640041
GTTTAAT84200.013.2292181
CTCGATT35450.012.99294951
GTCGAAT12350.012.9392711
TTTTTTG357150.012.9097022
AATCCAC109000.012.8064225
GTCGATT23250.012.7354841
CGAAAAT37250.012.6174511
Produced by FastQC (version 0.11.5)