FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21622067
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134250.042.6763594
CAAGTCG98950.024.36685494
TAAGTCG30900.020.53398194
GAAGTCG127300.019.08798294
GTTTTTT260300.015.7268551
CGCAAAC41950.014.3408831
AAAGTCG53300.013.66791794
AAAAATC173050.013.1724942
GTTTAAT81450.012.8103141
GCGAAAT31250.012.7841
CTCGATT37950.012.7562591
CGAAGAT80800.012.5643571
GTTTTAT139300.012.517591
AAATCCA185200.012.3083164
GAAAAAT181050.012.1231711
GGAAAAT150150.012.0512831
AAATCGA28150.012.0213164
CGCAATT21400.011.8598141
AATCCAA92500.011.838925
TTTAATC97450.011.7680872
Produced by FastQC (version 0.11.5)