FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22695977
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG97600.051.9118894
CAAGTCG82050.027.38086594
TAAGTCG26100.024.13026894
GAAGTCG112500.018.00622294
AAAGTCG44400.012.49099194
AATCGTA20450.012.1809285
ATCGTAT16400.012.0365856
GTTCGAT25750.011.8640781
GTCGGTT48700.011.5811091
GTCGATT22300.011.38116551
GGCGAAT57250.011.3292571
GTCGGAT27150.010.9060761
GTCGGAA35250.010.81
GTCGTTT21000.010.7428571
GTCGTAT11100.010.5855861
CGGGAAT54450.010.0991741
CGCGCAT14200.09.9295771
GTCCGAT15150.09.9273921
CGCGGAT22950.09.8300651
TAATCGT20600.09.8106794
Produced by FastQC (version 0.11.5)