FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22695977
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114500.044.0034994
CAAGTCG97200.024.85390794
TAAGTCG26450.023.10018794
GAAGTCG111650.017.175194
GCGGTAT20900.013.4928241
AATCGTA20500.012.38048655
ATCGTAT15700.012.2738866
AAAGTCG45550.011.96926594
GTCCGAT15000.011.9066671
CGCGGAT27100.011.7933581
GTTCGAT27450.011.47176551
TTCGATT28150.010.8525742
CGCGTAT4459.470328E-510.5617971
CGGGGAT76900.010.3289981
GTCGGTT45550.010.0087811
TTAGCGA17450.09.6962748
CGCATTT32400.09.4290111
TATACGG14900.09.30536834-35
CGTATCG14700.09.2721083
TCGGTTA14250.09.2350872
Produced by FastQC (version 0.11.5)