FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22000332
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG112750.057.2753994
CAAGTCG88200.028.50907194
TAAGTCG36800.026.18206694
GAAGTCG125000.019.852894
AAAGTCG47800.016.7154894
GTTTATT108600.012.8103131
CGAAAAT27950.012.7799641
CGGAAAT43550.012.6268651
TTAGCGA15900.012.4150937
CGAATCG18800.012.253
GGAAAAT181800.012.1765681
AAATCCA197950.011.7292254
GTCGGTT45150.011.5548171
CGCATAT8601.8189894E-1211.4767441
GCCGAAT49600.011.276211
GTTTTAT153100.011.2050951
CGCAAAT34150.011.1478771
CGCTAAT8056.184564E-1111.0931681
TCGAATC18000.010.9666662
GGCGAAT43350.010.9504041
Produced by FastQC (version 0.11.5)