FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22000332
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG132700.043.88319494
CAAGTCG103450.026.53262394
TAAGTCG37300.019.15281594
GAAGTCG121500.018.1810794
AAAGTCG52400.016.14503994
TACGCTA7150.013.1468539
GTCGGTT41350.013.07134151
GTTTATT113500.013.0026441
CGGAAAT39350.012.8996181
CGAATCG15500.012.7354843
CGCATAT9000.012.5333331
CGCAATT24400.012.3278691
CGAAGAT95300.011.984261
CGCGGAT27250.011.9009171
GGAAAAT175750.011.82021
GTCGTAT10750.011.8046511
CGGATTT35600.011.221911
GTCCGAT14750.011.1525431
CTTTAAT102950.010.7285091
TATACGC9651.8189894E-1210.7150257
Produced by FastQC (version 0.11.5)