FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23003406
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG70100.042.77603594
CAAGTCG57050.022.90271694
GTTTATT112100.019.1186431
TAAGTCG22850.018.71772694
GTTCGAT41000.017.3097551
TTCGATT41300.014.6803872
CAATCGG44250.014.020344
GTTTTAT173150.013.5720461
GTTTAAT98300.013.4354021
CGCGGGT66650.013.2573161
TATACGG28450.013.21616834-35
AATCGGG78350.013.0172315
ATATTAG41250.012.9890926
TAGCGAG33050.012.9409998
GTCGGTT61150.012.835651
CGCAATT24550.012.8268841
TTAGCGA35050.012.7389457
GTAAAAC71050.012.3040111
CGGAAAT49300.012.2981741
GTCGCAC33050.011.80332851
Produced by FastQC (version 0.11.5)