FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21762347
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106150.055.169194
CAAGTCG84600.027.16666694
TAAGTCG32000.024.52812694
GAAGTCG120700.021.68931294
TATACGC9500.019.2947357
TACGCTA9300.018.1935489
GTTTTTT251350.016.1372591
GTCGATT20350.015.0122851
ATATACG12650.014.4901186
CGAAAAT36350.014.3521311
AAATCGA29700.013.9259264
AAAGTCG45050.013.87569394
CGCAAAT29000.013.7758621
TTTTTTC318200.013.3230682
AAAAATC186650.013.2199312
GTTTAAT76950.012.94866851
GAAAAAT187650.012.82387351
CGAAAAC33100.012.7794561
CGGATAT22650.012.4503311
GTTTTTC155950.012.44693851
Produced by FastQC (version 0.11.5)