FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21762347
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG138500.042.58844894
CAAGTCG104200.024.71785294
TAAGTCG32350.022.66460694
GAAGTCG128600.019.00466594
GTTTTTT255150.015.6021951
CGAAAAT44350.013.88275051
CGAAAAC33250.013.7112781
TACGCTA8750.012.8914289
GCGAAAT27950.012.6118061
CGCGTAT4901.2754754E-712.4693881
GGAAAAT152850.012.1458951
CGCGGAT25600.012.11718751
AAATCCA185050.011.98811154
AAAGTCG52650.011.78347694
AAAAATC177850.011.7334842
CGGATAT22600.011.6460191
TTTAATC89600.011.5926342
CGCAAAC41100.011.5498781
CTCGGAT59000.011.3915251
GTTTTAT131150.011.28861
Produced by FastQC (version 0.11.5)