FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22735446
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG148250.049.61551394
CAAGTCG113450.027.3838794
TAAGTCG39250.024.3082894
GAAGTCG127050.021.30814694
GCGAAAT29450.017.2359921
CGCGAAT8550.016.4912281
AAAGTCG61700.015.31118394
AAATCGA34250.014.9576644
GTTTTTT305100.014.1415931
CGAATCG15850.013.9369093
CGAAAAC35450.013.52327251
GCGGAAT41400.013.3961351
GCCGAAT49950.013.36136151
AAAAATC223050.012.4532612
AATCCAC114550.012.4321255
CTCGATT37600.012.3751
AAATCCA201800.012.1110014
CGAAAAT46650.012.0900331
CGGAAAT47550.011.9600421
GAAAAAT227700.011.6209921
Produced by FastQC (version 0.11.5)