FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22191711
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG56400.022.16666694
TTAGCGA23500.013.68
AATCGTA25550.013.2446185
GTTCGAT31150.012.9759231
TAGCGAG28700.012.282239
GTCGGTT49000.012.2775511
CGCGGAT22000.012.1772731
TATACGG16100.012.11490734-35
CGCGGGT78850.011.6233351
GTCGAAA36850.011.4789691
CGCGTAA8207.2759576E-1211.4634141
GTCGTTT22150.011.4582391
CAAGTCG46050.011.43105394
TATACGC6654.431058E-911.308277
ATCGTAT21250.011.0588246
CGGGTCT119950.010.5794083
TCGGATT25800.010.383722
AAATCGA32200.010.3633544
GTTTATT109400.010.2248641
GTCCGAT14800.010.1621621
Produced by FastQC (version 0.11.5)