FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24495412
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG134800.055.01928794
CAAGTCG101500.029.68177294
TAAGTCG41750.022.17724694
GAAGTCG150550.019.98007494
AAAGTCG50000.016.63894
GTCGGTT55350.013.0767851
TCGAATC17550.012.85472
CGAATCG19100.012.5497393
GTTTATT118450.012.4989441
GTCGAAT13000.011.930771
GTCGATT24850.011.9154931
CGCAAAC46100.011.7245121
CGCGAAT16100.011.6770191
GTTTTTT346000.011.6549141
CGAAAAT33850.011.5243721
CTTTATT213350.011.4112961
GTTTTAT168900.011.4091191
CGAAAAC33800.011.2633131
CGGAAAT45100.011.2549891
GGAAAAT202350.011.218681
Produced by FastQC (version 0.11.5)