FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22147132
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG118950.056.8583494
CAAGTCG94250.030.11989294
TAAGTCG33350.023.53523394
GAAGTCG133400.021.8793194
AAAGTCG52250.017.1808694
GTTTTTT307550.014.8694531
TTTTTTC383250.013.0484012
CTCGATT37500.013.0346661
GGAAAAT176450.011.7733071
GAAAAAT229950.011.5481631
AAAAATC219150.011.4953222
CGAAAAT38150.011.3342071
TTAGCGA22000.011.3227277
GTTTAAT96100.011.2976071
GTTTTCT220950.011.23151
TTTTTTG445350.011.2289212
GTCGATT18600.011.1182791
CGCGAAT9300.011.1182791
GTTTTAT167250.011.0720481
AAATCCA197850.011.0224914
Produced by FastQC (version 0.11.5)