FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23043574
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG126100.040.5519494
TAAGTCG31100.023.27331294
CAAGTCG91100.022.7519294
GAAGTCG113400.017.36596194
GTTCGAT29300.017.1638221
TTCGATT33550.013.5886752
CGCGTAT4455.377169E-712.6741561
GTCCGAT15500.012.4322581
CGTAACG5152.4365363E-711.8640783
CGCGTAA6402.4283509E-911.7499991
CGGAAAT46300.011.6738651
TATACGG20400.011.28921434-35
TCGATTC37100.011.2749323
TCGGATT27400.010.9781022
AAAGTCG50100.010.60079994
CTCGGTT38100.010.4855641
GTCGTTT23550.010.3779191
GGCGATT40100.010.3142151
CGTATCG16500.010.2545453
CGGGAAT60500.010.1768591
Produced by FastQC (version 0.11.5)