FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22979902
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG109300.054.00914894
CAAGTCG94050.029.68421294
TAAGTCG31100.028.10932494
GAAGTCG126200.019.10538794
AAAGTCG46550.013.73147294
GTCGTTT18800.013.2500011
GTTCGAT26500.012.9471691
TTAGCGA25800.012.2054268
GCCGATT22800.012.1622811
TAGCGAG28750.011.9339139
GTCGGAA40300.011.6625321
GTCGAAT13600.011.0588241
GGCGAAT56800.010.9225351
CGTATCG19000.010.884213
CGCATAT8802.910383E-1110.6818181
GTCGAAA37900.010.66490651
TCGCGTA5751.0123767E-610.6260862
TAACGCA24800.010.61290452-53
CGCGGGT78800.010.4974621
GTCCGAT15750.010.4444441
Produced by FastQC (version 0.11.5)