FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22173661
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104850.051.77396494
TAAGTCG29700.029.43434594
CAAGTCG79700.026.94981494
GAAGTCG122600.021.73654294
AAAGTCG46500.016.77849694
GTTTAAT94900.012.6290841
GTCGGTT45100.012.6097561
CGGAAAT45200.011.9579641
CGCATAT10250.011.9219511
TCGGATT26350.011.5939282
GGAAAAT176200.011.5766181
CGAAGAT81000.011.5469131
GTCGATT19750.011.4227861
AAATCGA32700.011.3547414
GTTTTTT281750.011.2766651
CGAATCG14900.011.0402693
GTCGGAT28400.010.9225361
GTTTATT104500.010.8842121
CGCGAAC14600.010.6232881
AATCGTA20050.010.5486295
Produced by FastQC (version 0.11.5)