FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23021899
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG100250.030.56758394
TAAGTCG27700.018.15523594
CAAGTCG76300.017.740594
GTCGGGT51250.017.5160981
CGCGAAT8500.016.5882361
TCGGGTC47900.015.306892
CGCGTAA6800.015.2058831
CGAATCG14250.014.5122813
GTAAAAC75800.014.1992091
GAAGTCG91500.013.86885394
CGCGAAC8400.013.4285721
GTCGGTT55800.013.3082441
GTTTATT127650.013.2549951
AATCGTA20050.013.1271845
GTCGTTT23600.012.7457631
GTTCGAT44650.012.5263171
ACGCTAC29700.012.5016845
GACGCTA29200.012.3938364
CGCGTAT4203.8950875E-612.3095251
CGAAAAT59100.011.8494081
Produced by FastQC (version 0.11.5)