FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21717616
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127650.057.18057694
CAAGTCG96350.032.0975694
TAAGTCG38000.027.58157794
GAAGTCG151100.021.52481794
AAAGTCG49800.017.55421894
CGAAAAT33400.014.775451
TACGCTA8400.013.4285729
ATATACG10100.013.0297026
GTTTTTT268400.012.0827121
CGCATAT8750.011.8171431
CGAAGAT84050.011.7989291
GTTTATT97800.011.6779141
GTTTAAT85350.011.5641481
CGCAAAT31300.011.56231
TTTTTTC326900.011.5307442
GTCGGTT47000.011.31
CGAATCG14200.011.2535223
GTTCGAT33100.011.2175231
CTTTATT159750.011.209391
TCGGTTA12300.011.0813012
Produced by FastQC (version 0.11.5)