FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21246308
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC8900.017.9550557
GTTTTTT314300.017.914731
AAGTCGG75800.017.8575294
TACGCTA9600.017.1354169
CGAAAAC38000.016.8210531
CTCGATT38700.016.1524541
GTTTTAT163150.015.0665021
CTCAGAT169500.014.7793521
CTTTAAT128600.014.6555211
AAATCGA32300.014.4055724
GTTTAAT96700.014.1923481
CTTAGAT55900.014.1252241
CTTTGAT172750.014.0387851
CTTTTAT151550.013.8627511
GTTTTTC179800.013.74972251
TCGATTG21200.013.7452832
ATATACG13100.013.6335886
CGAAAAT39350.013.6162641
CGGATAT20600.013.4611661
CGCAATC25000.013.3481
Produced by FastQC (version 0.11.5)