FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22472495
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG98750.050.4982394
CAAGTCG78200.026.02429694
TAAGTCG33100.022.29305394
GAAGTCG119800.017.9682894
CGAAAAT36500.014.5506861
TATACGC8750.013.4285727
AAAGTCG47050.012.78639894
GTCGGTT51100.012.7847381
TACGCTA8500.012.7176489
CGCAAAT30850.012.1880071
GTTTATT102850.011.8813811
TCGAATC20350.011.7788712
CGCGTAT5605.6908902E-811.7500011
GGAAAAT171900.011.64746951
GTTTTTT286750.011.4242371
GTTTTAT149300.011.1754871
CGGAAAT44550.011.0774411
ATATACG11550.010.9870146
GTCGAAT14550.010.9828181
GGCGAAT55150.010.9084321
Produced by FastQC (version 0.11.5)