FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22013527
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG80600.043.55955594
TAAGTCG31850.020.06907594
CAAGTCG59100.019.24534694
CGGATAT24100.015.79667951
GTTTTTT316750.015.6839761
GAAGTCG100800.015.62003994
TACGCTA10550.015.1469199
TTTTTTC491500.015.0132252
TATACGC11650.014.12017257
CGAATCG17300.013.8554923
CGAAAAT33250.013.5699251
ATATACG13900.013.525186
GTTTTAC94200.013.1220811
AAATCCA202750.012.888784
CTTAGAT84850.012.7955211
TCGGATC44650.012.7368422
TTTTTTG549200.012.6828122
CGCAAAT26050.012.62955951
CGAAAAC35000.012.2199991
CGAAGAT68950.012.2015951
Produced by FastQC (version 0.11.5)