FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305406

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305406
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22013527
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG106500.033.53990694
CAAGTCG75550.020.4050394
CGGATAT23900.015.928871
GTTTTTT295800.014.8563211
CGAAAAC42800.014.27570151
CGAATCG16850.014.2255183
CGCGTTT13750.014.0145451
CGCAAAT28950.013.9620041
GAAGTCG100300.013.63609294
CGAAGAT71250.013.5887711
CTCAGAT181950.013.5097541
CGAGAAC61900.013.1357031
CTCGATT37250.012.8697981
GTTTAAT100200.012.7584831
TAAGTCG35850.012.71687594
AAATCGA33150.012.4766214
CTTAGAT78650.012.4297511
CTCGGAT69900.012.371961
CGAAAAT36900.012.2276421
CGCAAAC40050.012.0873911
Produced by FastQC (version 0.11.5)