FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18084112
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104600.036.17112794
CAAGTCG79800.022.96992594
TAAGTCG24950.020.53306694
GAAGTCG95700.017.53291594
GTCGTTT16750.014.3104491
CGAAAAT35900.014.2701961
CTTAGAT48550.014.0370751
GTTTTTT232800.013.8698441
CGCAATT16000.012.9251
CGGATAT17650.012.781871
CTCGATT33200.012.7409641
CGCAAAT28500.012.6982461
TACGCTA7800.012.6538469
GGAAAAT133900.012.4256921
GTTTAAT73650.012.2525461
CGAAAAC32300.012.0773991
CGAATCG13800.011.920293
AAATCCA162900.011.7139344
TATACGC6452.743036E-911.6589157
GAAAAAT177950.011.6476541
Produced by FastQC (version 0.11.5)