FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21729659
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG121650.054.12824294
CAAGTCG94850.028.93832694
TAAGTCG37300.021.92493294
GAAGTCG131050.021.91301294
AAAGTCG48200.015.69917194
CGAAAAT33350.014.9385311
GTTTTTT269950.014.3985941
GTCGAAT13800.013.2826091
CGCGAAT12950.013.0656391
GTTTAAT81100.012.9815051
TTTTTTC345650.012.9176922
GTTTTAC64950.011.8675911
CGAATCG16250.011.8584623
GTTTTAT143350.011.6721321
AAATCCA189450.011.4864094
CGCGTAT4104.116115E-511.4634151
AATCCGC22700.011.3876655
AATCCAC109350.011.2610895
CGCAAAT29800.011.1979881
GTTTTCT208750.011.144911
Produced by FastQC (version 0.11.5)