FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23771532
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124350.056.1656694
TAAGTCG33450.030.6307994
CAAGTCG99500.028.3417194
GAAGTCG139350.023.13742494
AAAGTCG49650.016.471394
GTTTTTT290200.015.67746451
TTTTTTC361250.014.9098962
CGAAAAT40050.012.9088641
GAAAAAT217850.012.4916231
GTTTAAT91550.012.32113551
TATACGC8400.012.3095247
TTTTTTG403150.011.9846212
GTTTTAT166350.011.979561
GTTTTCT224200.011.7814451
CGCAAAT30950.011.6930531
AAATCCA199650.011.6528924
CGAATCG15350.011.63517953
AAAAATC209050.011.6010522
TTAGCGA24400.011.5573787
ATATACG12200.011.5573786
Produced by FastQC (version 0.11.5)