FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21609767
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG124200.056.11996594
CAAGTCG96600.028.60869494
TAAGTCG36900.026.62059494
GAAGTCG134400.022.66071394
GTTTTTT277250.016.8844011
AAAGTCG49700.015.98189194
TATACGC10650.013.680757
CTCGGAT66800.013.43862151
CGAAAAT34100.013.3695011
GTTTAAT89600.013.323661
TTTTTTC345500.013.1409542
TACGCTA11300.012.8938059
CGCAATT21300.012.3568071
TCGGATC43150.012.3082282
GCGAAAT27550.012.2831211
CTCAGAT154250.012.1270651
AAATCGA30000.011.9066674
GTTTTAT153950.011.8148741
AAAAATC204500.011.7902182
AAATCCA190350.011.7777784
Produced by FastQC (version 0.11.5)