FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23048251
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG92750.044.6436794
CAAGTCG76600.021.22976794
TAAGTCG30450.019.29392694
GAAGTCG114050.015.8246494
GTTTTTT310850.014.8930351
TACGCTA8850.014.8700579
TTTTTTC447350.014.76137352
TATACGC9300.014.6559157
GCGAAAT29850.014.1708551
TTTTTTG481500.013.1482882
CGAATCG17550.013.1225083
AAATCGA36350.012.5419544
CGGAAAT43700.012.3684211
GAAAAAT224600.012.262691
GGAAAAT186500.012.222521
GTTTAAT90700.011.9184131
GTTTTTC181050.011.8635741
CTTAGAT63400.011.8611991
TTTTTTA432550.011.77852252
CGAAAAC37750.011.7033121
Produced by FastQC (version 0.11.5)