FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20420832
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG85650.050.92352794
CAAGTCG69950.024.18870594
GAAGTCG97950.020.05717394
TAAGTCG27750.018.894
GTTTTTT299850.018.2607981
CGAAAAT35400.015.0028241
TTTTTTC376200.014.7171712
TTTTTTG413500.013.6169292
CTCAGAT150100.013.1512331
CGCGAAT12550.013.107571
GTTTAAT103150.012.9859431
AAAGTCG46750.012.96898494
GTTTTAT166250.012.7500751
AAATCGA30600.012.7483654
AAATCCA195050.012.7469884
CTCGATT40800.012.6715691
GTTTTCT226250.012.5679561
CGCAAAC38900.012.444731
CGAAAAC29100.012.4364251
CGCAATC21300.012.3568071
Produced by FastQC (version 0.11.5)